ABSTRACT
In this work, we reporta simple and green approach method for the green synthesis of stable silver nano particles (AgNPs) using syzigium cumini leaf extract. Leaf extract acts as both reducing and stabilizing agent. The synthesized nanoparticles were characterized using UV-visible spectroscopy (UV-vis), Fourier transform electron microscopy(FTIR), X-ray powder diffraction (XRD), Scanning electron microscopy(SEM), zeta Potential and Transmission electron microscopy (TEM).The synthesized AgNPs was characterized by a peak of 439- 441nm.The influence of reaction time on the synthesis of nanoparticles were studied. Zeta potential value -14.1shows a moderate stable of silver nanoparticles with Syzigium cumini leaf extract. The TEM image clearly shows the synthesized silver nanoparticles shape is spherical and the average size is 13 nm. Synthesized nanoparticles had significant anti-bacterial activity and it also shows good catalytic activity on the reduction of para nitro phenol (4- NP) to para amino phenol (4-AP).
ABSTRACT
A method has been developed for biospecific interaction analysis between antigen and antibody using solid phase binding approach. Real time kinetics between monoclonal antibody and human chorionic gonadotropin have been studied. Kinetic constants of the bimolecular reaction are determined. Affinity constants measured by several independent methods have been found to be relatively consistent. Convenient and simple procedures to determine affinity constant, Kon and Koff of monoclonal antibody-human chorionic gonadotropin interaction using binding of [125I]hCG to immobilized monoclonal antibody are presented. Values obtained compare well with those obtained using surface plasmon resonance technology,making this method a viable alternative.
ABSTRACT
Two hundred and fifty stool samples were studied during an outbreak of cholera in Goa during the months of July to September, 1988. 80 strains of Vibrio were isolated with an isolation rate of 32%. 72.5% of those affected were adults. All strains of Vibrio cholerae isolated belonged to Eltor biotype, Fifty three (66.25%) of them being Ogawa serotype while 21 (26.25%) were Inaba. NAG Vibrios accounted for 6 (7.5%) strains. Antimicrobial sensitivity pattern showed high degree of sensitivity to chloramphenicol, gentamicin and naladixic acid.